However, metal stent has no curative function, and tumor-overgrowth inside stent normally induces biliary obstruction. Since bile duct is blocked by tumor growth or inflammation, stent displacement is frequently employed to prevent bile duct occlusion and to prolong patient survivability amongst aforementioned treatments. Various treatment options such as radiotherapy, chemotherapy, metal stent displacement, and immune-adjuvant therapy have been tried to treat CCA patients. Since most of the CCA patients are frequently diagnosed in advanced state, very few cases of the CCA patients are amenable to surgical resection. The reason of carcinogenesis and increase in incidence rate are still unclear even though its incidence rate is increasing over worldwide. We suggested PL-incorporated ROS-sensitive nanofiber mats as a promising candidate for local treatment of CCA cells.Д¬holangiocarcinoma (CCA), which is normally derived from the bile duct region, is regarded as one of the most aggressive cancers. PL-incorporated ROS-sensitive nanofiber mats were coated onto GI stent and showed improved anticancer activity with ROS responsiveness.

In HuCC-T1 cell-bearing mice, PL-incorporated nanofiber mats showedВ improvement in anticancer activity. Furthermore, PL released from nanofiber mats properly produced ROS generation and induced apoptosis of CCA cells as well as PL itself. PL itself and PL released from nanofiber mats showed almost similar anticancer activity against various CCA cells. Nanofiber mats composed of PCL/LEse polymer blend showed ROS-sensitive drug release, i.e., PL release rate from nanofiber mats was accelerated in the presence of hydrogen peroxide (H 2O 2) while nanofiber mats of PCL alone have small changes in drug release rate, indicating that PL-incorporated nanofiber membranes have ROS responsiveness. The higher amount of LEse in the blend of PCL/LEse resulted in the formation of granules while PCL alone showed fine nanofiber structure. Various ratios of poly(Оµ-caprolactone) (PCL) and LEse block copolymer were dissolved in organic solvent with PL, and then nanofiber mats were fabricated by electro-spinning techniques. Selenocystamine-conjugated methoxy poly(ethylene glycol) (MePEG) was conjugated with poly(L-lactide) (PLA) to produce block copolymer (LEse block copolymer). A ROS-producing agent, piperlongumine (PL)-incorporated nanofiber mats were investigated for drug-eluting stent (DES) application.

The aim of this study is to fabricate drug-eluting gastrointestinal (GI) stent using reactive oxygen species (ROS)-sensitive nanofiber mats for treatment of cholangiocarcinoma (CCA) cell.

Stent using a guideliner